Structural Measurements of peptides and bio-macromolecules
The greater the knowledge regarding the structure and functional groups of a peptide, the more precise the predictions concerning its mode of action. NMR Spectroscopy is the only method that enables a complete structural analysis to be made of dissolved peptides.
It is possible to measure peptides containing in excess of 100 amino acids using one-dimensional (1D-NMR), two-dimensional (2D-NMR) or three-dimensional (3D-NMR) methods. Special effects such as pH-dependence, dissolving behaviour etc., can clearly be shown. Furthermore, the complete three-dimensional structure of most peptides can be determined.
In addition to peptides other bio-macromolecules such as nucleic acid etc. can be measured by NMR spectroscopy. Thus, structural analyses of Human Immunoglobulin were performed successfully by using labelled 13C and 15N.